Page 27 - PCC02

6 5 4 3 2 1 0
Differences in content of non‐declared protein between manufacturers and between batches
Dyrlund et al., 2014
Vitrolife
HSA Vit G1P G2P
Vitrolife
COOK
ClevageL1 Clevage L2 Blast L1 BlastL2
EA
BA SegClev Seq Blast
COOK
ART culture media Growth factors
Origio
• Insulin
– Added in very high doses by some
media manufacturers • GM‐CSF
– Granulocyte‐ Macrophage Colony‐ Stimulating Factor
– Added to media by one manufacturer
• Necessary, Beneficial?
– Will change methylation pattern
in mouse embryos • Reduce apoptosis?
– May increase likelihood of delivery in recurrent abortions?
– Effect on the epigenome?
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 27
% of protein content