Page 90 - Eshre 2018

The CRISPR‐Cas9 system can mutate genes in SCCs. The mutated SSCs underwent spermatogenesis after transplantation into the seminiferous tubules of infertile mouse testes, resulting in production of heterozygous offspring displaying the corresponding mutant phenotypes. Furthermore, a disease‐causing mutation in genes could be readily repaired, resulting in SSC lines carrying the corrected gene with no evidence of off‐target modifications with 100% efficiency.
90
Page 84 of 130