Page 92 - Eshre 2018
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How to target the female germ line?
Relatively easy access to oocytes
Small numbers, needs to be very efficient Will it work in meiosis?
Later embryo selection
CRISPR in oocytes...
MII oocyte microinjection of CRISPR‐Cas against PDX1, a master regulator of pancreas development reduced lysis, improved blastocyst rate, increased the number of targeted bi‐allelic mutations, and resulted in similar degree of mosaicism when compared to zygote microinjection.
Complete PDX1 disruption was achieved, resulting in a PDX1−/− fetus that lacked a pancreas.
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