Page 131 - Eshre 2018
P. 131
>
Part III: Conclusion
25.05.2018
Prof. Dr. Eckart Meese Seite
21
>
Part IV: Materials and Methods
Overview of the design strategy
Purification of Extracellular
Total RNA isolation
miRNA microarray
Real Time PCR
Data analysis
Oligoasthenozoospermia
Seminal Plasma-derived extracellular microvesicles
Normozoospermia
Western Blot Confirmation
Objective: To determine whether microRNA (miRNA) expression profile is different in extracellular microvesicles collected from seminal plasma of men with oligoasthenozoospermia.
01.07.2018
Prof. Dr. Eckart Meese Seite
22
Page 125 of 130
PRECONGRESS COURSE 01 I BARCELONA, SPAIN – 1 JULY 2018 131