Page 130 - Eshre 2018
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Part III: Materials and Methods
Overview of the design strategy
Spermatozoa Testicular tissues
Total RNA isolation
RNA Assessment
Real Time PCR
Data analysis
Subfertile males (n= 80)
Fertile males (n= 90)
Testicular impairments (n= 48) Control males (n=16)
Objective: To validate a set of five miRNAs as specific biomarkers for the assessment of male infertility (miR-34b* . miR-34b. miR-34c-5p. miR-429. and miR-122)
01.07.2018
Prof. Dr. Eckart Meese Seite
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Part III: Results
Support vector machine classifications
Comparison
Classification
Accuracy
Specificity
Sensitivity
Subfertile vs. Control
98.65 % ± 0.27
98.83% ± 0.25
98.44 % ± 0.56
NOA vs. Control
99.91% ± 0.4
99.69% ± 1.4
100% ± 0.00
25.05.2018
Prof. Dr. Eckart Meese Seite
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