Page 109 - Eshre 2018
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Small non‐coding RNAs in spermatogenesis
mRNA
mRNA 5’
• 17 to 35 nucleotides long
• Base pair with target RNAs (usually mRNAs)
• Fully complementary or partially complementary
• Involved in the post-transcriptional regulation of gene expression • May also function at the transcriptional level
miRNAs - processed frommiRNAprecursors by DROSHAand DICER endo-siRNAs –processedfromendogenousdsRNAbyDICER piRNAs - processed frompiRNAcluster transcripts
tsRNAs - processedfromtransferRNAs(tRNAs)
5’
Fully complementary
3’
3’
siRNA
5’
Partially complementary
miRNA Seed 5’
microRNAs (miRNAs)
• miRNAsareexpressedinacell-specificmannerduring spermatogenesis, they participate in the control of each step of male germ cell differentiation
• Geneticallymodifiedmousemodelshavedemonstratedthe importance of miRNA pathways for normal spermatogenesis
• HumanspermatozoalorseminalplasmamiRNAshavebeen explored as potential biomarkers for male factor infertility
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PRECONGRESS COURSE 01 I BARCELONA, SPAIN – 1 JULY 2018 109
