Page 70 - PRECONGRESS COURSE 04

In-vitro models – Pros and Cons
+ • -
low costs / good availability
• use of human cells possible
• evaluation of single parameters
• activation / inhibition of special pathways
• analysis of epithelial stromal interaction
• artificialsystem
• isolatedcellsimportantfactorsmaybemissing
• results may depend on use of 2D or 3D culture systems • resultshavetobeverifiedin-vivo
Do in‐vitro models show potential for translational application?
• It is possible to induce states of increased or reduced endometrial receptivity in‐vitro.
• The models are useful in understanding factors that regulate endometrial receptivity.
University Hospital Essen - Prof. Dr. Ruth Grümmer ESHRE 2017 - Geneva
Summary
+ closest to the human situation
‐ costly, limited availability, ethically challenging
+ similar features of implantation compared to human
+ many different and sophisticated experimental approaches feasible
+ low costs / good availability
‐ Possible differences in reproductive physiology –
results have to be verified for the human situation
+ low costs / good availability use of human cells
+ different experimental approaches possible
‐ artificial system
‐ results have to be verified in‐vivo
University Hospital Essen - Prof. Dr. Ruth Grümmer
ESHRE 2017 - Geneva
70