Page 36 - PRECONGRESS COURSE 04

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3. Mechanisms & endocrine control 3.1. Animal models (rodents)
LH
ERα NULL MICE
• The endometrium is un‐receptive Proestrous 1 2 3 4
5 6
days of pregnancy
degeneration luminal epithelium
• PRAinhibitory estr
PR • PRBno role in fertility
roliferative effect
stroma cell decidualisation
21
PRE-RECEPTIVE
WOI
Post-receptive
progestrone
estradiol
PR NULL MICE (PRAKO / PRBKO)
Luminal epithelium
• Infertile; defect in blastocyst implantation
• no decidualisation Stroma cells
Robertshaw, et al. 2016 Measuring and enhancing endometrial Wang and Dey 2006, Zhang, et al. 2013 receptivity to improve pregnancy outcomes
3. Mechanisms & endocrine control 3.1. Animal models (rodents)
Muc-1
a
d
C
l
p
O
PR
ERα
U
i
o
P
T
ERα
Estrogen specifies the duration of the WOI in mice
Ma et al. 2003
They use OVX mice prior to implantation
• estradiol is necessary in progesterone primed uterus
• too low dose of estradiol does not support implantation
• too high dose makes the endometrium unreceptive
• Varying doses changed the WOI duration confirmed by receptivity molecular markers
Measuring and enhancing endometrial receptivity to improve pregnancy outcomes
22
F
II
embryo