Page 68 - PRECONGRESS COURSE 03

SOME EXAMPLES OF COMPLETE FAILURE CASES
Oocyte Maturation Blocks
M-I M-II
Spontaneous Activation
2 PN, 2 PB
+
GV
Combelles, 2012
X
Oocyte Activation Failure (sperm and/or oocyte defect)
Spermiogenesis and/or Centrosome Defect
A CASE OF SPONTANEOUS ACTIVATION
Light microscopy evaluationAbn. cleavage patterns SNP microarray and PCR  Maternal origin
FISH  Aneuploidy
Cytoskeletal and chromatin analysisSpontaneous activation
Chromatin
Microfilaments
Microtubules
• Whatelsecouldbedone?
• Follow-upanalysesofthedefects→susceptiblegenetargets
• e.g.identificationofcandidatevariantsbywhole-exomesequencing
➾Oocyte- specific defect with failure to arrest in M-II
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Combelles et al., 2011