Page 67 - PRECONGRESS COURSE 03

FERTILIZATION FAILURES
ETIOLOGIES
20-23% (IVF/ICSI) PN formation,
6-11% (IVF/ICSI)
57% (IVF)
dynamics
penetration
sperm sperm aster
6% (IVF)
formation
activation
sperm decondensation
8% (IVF/ICSI)
40% (ICSI)
Asch et al., 1995 Rawe et al., 2000
FERTILIZATION FAILURES
DIAGNOSTIC EFFORTS
• Assessment of the structure and function of gametes
• Semen analysis
• Bioassays
• Human sperm-oocyte interaction tests
• Sperm-ZP binding
• Human ZP-induced acrosome reaction • Sperm-ZP penetration
• Sperm-oolemma binding ratio
• Sperm penetration test using ZP-free hamster oocytes • Sperm aster formation test in bovine or rabbit oocytes
• Need to include assessments beyond morphology • To detect sub-microscopic lesions
• While considering feasibility within a clinical setting
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PRECONGRESS COURSE 03 I GENEVA, SWITZERLAND – 2 JULY 2017 67