Page 70 - PRECONGRESS COURSE 03

ACTIVATION DEFICIENCIES
• Scope of the defect
• 40% of FF cases after ICSI (Rawe et al., 2000)
• By early 2015, a systematic review identified 47 papers on the effectiveness of assisted oocyte activation(AOA) during ICSI (Rubino et al., 2016)
• Mounting body of investigations and evidence
DIAGNOSTIC AND PROGNOSTIC ANALYSES
• Testing of parental origin
• Mouse oocyte activation test (MOAT)
• Low activation (sperm defect), intermediate, or high activation (oocyte defect)
• Mouse oocyte calcium analysis (MOCA) • Scoring of calcium oscillatory patterns
• Split fertilization
• Sibling oocytes with either partner’s or donor
sperm
• Identification of the molecular defect
• From expression, sequencing, and functional analyses
PLC- Ca2+ oscillations
Ca2+ response machinery Activation
• Sperm issues in activating ability
• Phospholipase (PLC) zeta expression, forms,
localization, and mutations
• Impairments in oocyte response system
• Not yet identified specifically
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