Page 127 - PRECONGRESS COURSE 01

Investigation on the origin of sDF in humans: an ex-vivo study
• Direct association of sDF with: Markers of apoptosis
(caspase activity and cPARP)
Markers of oxidative damage
(8-OH guanosine and malondialdehyde)
Marker of chromatin immaturity (anyline blue staining)
Muratori et al, Mol Med, 2015 Marchiani et al, Andrology 2014
Direct association TUNEL/Caspase activity
AB
UL UR 1.1% 17.4%
100
80
60
40
***
LL LR 20
10.0%
0
C
FLICA
CASPASE ACTIVITY
TUNEL
Fragmented Non-Fragmented Sperm Sperm
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 127
sDF
Caspases activity (%)