Page 125 - PRECONGRESS COURSE 01

Learning objectives
1. Types of DNA damage found in spermatozoa
2. Mechanisms of DNA fragmentation in spermatozoa: results of an ex‐vivo study
3. Techniques to evaluate sperm DNA fragmentation
4. Consequence of sperm DNA fragmentation for natural and assisted reproduction
5. Effect of in vitro manipulation of spermatozoa on sperm DNA fragmentation
6. Who can benefit from DNA fragmentation evaluation?
Main types of damage to genetic material found in sperm
Genetic damage
- Mutations
- Deletions
- DNAstrandbreaks
- base oxidation
- Chromosome aberrations (aneuploidies)
Other damage that can be transmitted to the progeny
- Epigenetic damage (DNA methylation)
- extentofprotamination
- Histone retention
- Statusofsulfhydril groups
- Significance of sperm RNA (and miRNA)
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 125