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Washed sperm preparation
 Simultaneous imaging and analysis of multiple sperm trajectories
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CASA Imaging Issues
Examples:
 Including debris as spurious sperm heads: can increase concentration and decrease % motile and % progressive
 Rejecting clumped (usually dead) sperm that digitize as a contiguous object that is “too big” to be a sperm: can reduce concentration and increase % motile and % progressive
 “Stirred” immotile sperm (real or spurious): can increase % motility, and decrease % progressive
 Tracking of sperm through collisions (real or perceived) can cause track fragmentation: can increase concentration and increase % motile and % progressive
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 111