Page 42 - ESHRE2019
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2011
•Generation of a novel differentiating 2 step- protocol without overexpressing any genes.
•iPS can form in vitro meiotic and haploid like- cells.
•The post-meiotic-like cells generated have features of human spermatids expressing Acrosin.
2011
• Generation of in vitro PGCs from hESC and hiPS over- expressing VASA and/or DAZL.
• The hES/hiPS can form correctly in vitro meiotic and post-meiotic male haploid cells over-expressing VASA and/or DAZL in 14 days.
• The in vitro germ-like cells over- expressing only VASA present the typical pattern of methylation status for H19 as the normal germ cells.
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