Page 38 - ESHRE2019
P. 38
2016
• Here they report complete in vitro meiosis from embryonic stem cell (ESC)-derived primordial germ cells (PGCLCs).
• Co-culture of PGCLCs with neonatal testicular somatic cells and sequential exposure to morphogens and sex hormones reproduced key hallmarks of meiosis, including erasure of genetic imprinting, chromosomal synapsis and recombination, and correct nuclear DNA and chromosomal content in the resulting haploid cells.
• Intracytoplasmic injection of the resulting spermatid-like cells into oocytes produced viable and fertile offspring, showing that this robust stepwise approach can functionally recapitulate male gametogenesis in vitro.
• These findings provide a platform for investigating meiotic mechanisms and the potential generation of human haploid spermatids in vitro
36
33
