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New experimental design - IVF laboratory
Prospective pilot study Igenomix-Genera (November 2017 - March 2018)
in 115 blastocysts to define optimum time for media collection with new protocol
Standard culture
D4 embryos
Serial Washes
Washings
Transfer to a 10 μl drop
10 μl drop
Cell-free DNA
Culture up to Day-5/Day-6/Day-7
D5/D6/D7 biopsy
Optimization to minimize Maternal DNA contamination
Rubio et al., ESHRE 2018 (Submitted F&S 2019)
New NGS protocol
Modified Reproseq protocol <15 h Ion Reporter 5.4
Ion TorrentTM Technology (S5)
WGA with conventional and modified protocol
After 5 minutes
Time in culture:
CMCM
30h 30h
CMCMCMCM
30h 48h 48h 72h
Rubio et al., ESHRE 2018 (Submitted F&S 2019)
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PRECONGRESS COURSE 10 I VIENNA, AUSTRIA – 23 JUNE 2019 131
