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Our previous experience in niPGT-A
WGA
Day-5 blastocyst
12 embryos
NGS
WGA
Concordant
30.4%
17 17 17 5
Partial maternal contamination 30.4%
Full maternal contamination 30.4%
Non-informative
8.9%
Previous studies of cell-free DNA in spent culture media
PGT-A
Vs. niPGT-A
3.5% concordance (N=55)
27% concordance (N=22)
30.4% concordance (N=56)
65.0% concordance (N=61) 85.7% concordance (N=42) 87.5% concordance (N=47) 75.0% concordance (N=40) 84.0% concordance day-6 (N=81)
Shamonki et al., Fert&Ster 2016 Feichtinger et al., RBMonline 2017 Vera et al., HR 2018 Ho et al., Fertil Steril 2018 Xu et al., PNAS 2016 Kuznyetsov et al., PlosOne 2018 Li et al., Scientific Reports 2018 Rubio et al., ESHRE 2018
High variability between results
Embryo mosaicism
Maternal DNA contamination
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