Page 118 - ESHRE2019

Post transcriptional gene silencing
• “Seed” region = binding site
o 7-8 nucleotides
o 5’-end of miRNA strand
o Complementary with target mRNA
• 3’-end base paring
o Weaker
o Less important
Short binding site
↓
1 miRNA = several mRNAs
1 mRNA = several miRNAs ↓
Complex regulatory pathways
miRNA as biomarker
• miRNA is present in every tissue
• miRNA is also present in body fluids o Blood
o Urine o Saliva o...
• Circulating miRNA = stable o Exosomes
o Protein bound
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