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DNA Typing Resolution
• Low
– serologic equivalent – DRB1*04, DRB1*13
• Intermediate
– DRB1*04:01 or *04:03 or *04:04... – DRB1*04:01/03/04
• High
– DRB1*04:01
– DRB1*04:01/36/45/52/56
• NGS: allele level
ambiguities
Serologic typing of HLA antigens Microlymphocytotoxicity test
Anti-HLA antibodies
Positive reaction
Negative reaction
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PRECONGRESS COURSE 10 I BARCELONA, SPAIN – 1 JULY 2018 29