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Nomenclature
HLA
HLA-A HLA-A*02 HLA-A*02:01 HLA-A*02:01N HLA-A*02:01:01
HLA-A*02:01:01:01 HLA-A*02:01:01L
HLA complex Locus
Ag equivalent Allele specificity Null allele
Silent mutation (coding region) Noncoding region mutation
Low expression gene
HLA Typing methods
Past
-Serologic typing of HLA antigens Microlymphocytotoxicity test
-Cellular assays
Mixed lymphocyte culture
Present (DNAbased)
-Sequence Specific Oligonucleotide (SSO) -Sequence Specific Primer (SSP) -Sanger-sequencing Based Typing (SBT)
Future
- Next-Generation Sequencing (NGS)
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