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Embryonic stem cells
Derived from the inner cell mass of the blastocyst (ICM) (day 5- 7 of development,
150-200 cells). They give rise to the 3 germ layers: ectoderm, mesoderm and endoderm.
PCC Stem Cells - ESHRE BCN 2018
Induced pluripotent stem cells (iPS) generation
• The first iPS cell line was generated with 24 factors. (Takahashi K & Yamanaka, 2006)
• The Classical 4 factors cocktail: Oct4/3,Sox2,c-myc&KlF4 or
O c t 4 / 3 , So x 2 , Li n 2 8 & N a n o g (Takahashi K & Yamanaka S., 2006, Takahashi K et al, 2007, Yu et al, 2007Park et al,2008)
PCC Stem Cells - ESHRE BCN 2018
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Stable Karyotype Methylation of Nanog/Oct4 promoters Transgene expression silencing Expression of endogenous pluripotent associated markers
In vitro/ In vivo differentiation Chimera contribution*
PRECONGRESS COURSE 10 I BARCELONA, SPAIN – 1 JULY 2018 11
