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Gene correction. Rat Model. Spermatogionial Stem Cells
• Use of CRISPR/Cas9 to target heritable mutations within rat spermatogonia.
• Clonally enriched target alleles were vertically transmitted from donor spermatogonia to rat progeny.
• Directgermlineeditingin spermatogonia prevented CRISPR/Cas9-catalyzed animal mosaicism by avoiding totipotent and pluripotent states of embryogenesis.
Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells Chapman,2016
PCC Genetics - ESHRE BCN 2018
Germline genome editing
DNA editing of germline can be performed in
1. Stem cells
• Pluripotent stem cells differentiated into gametes to
further give rise to an embryo (in vitro gametogenesis –
IVG)
• Spermatogonial stem cells 2. Zygotes or Embryos
PCC Genetics - ESHRE BCN 2018
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