Page 75 - PCC02
P. 75

 Learning objective
• Appreciate how the IVF culture system, as a whole, has significant margins of improvement irrespective of founding decisions, such as the choice of specific culture media
 Outline
• Gametes handling/incubation
• Timing (IVF/ICSI) and duration (IVF) of insemination
• (Culture media per se not covered, topic of previous talk) - Protein source
- Ammonium - Oil
• Oxygen tension
• Temperature
• Culture system technicalities - Culturedrops
- Individual/groupculture - Light
• Manipulation (Biopsy)
• ...... List not exhaustive of the topic
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