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Conclusions
• Optimization of culture media formulations by continued exploration into gamete and embryo phsiology is of critical importance for the success of ART
• However, what’s evident is that media are but only one aspect of the embryo culture system, which comprises other factors, both chemical and physical
• One of the MOST important tasks of an embryologist is to be conscious of how human gametes and embryos can be sensitive to such factors, and thus have an adequate QC&QA program in place for optimizing performance and successfully maintain high pregnancy outcome
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1. Morbeck DE et al., Composition of commercial culture media used for human embryo culture. Fertility and Sterility Vol.102, No. 3, pp.759-766 e9, 2014
2. Dardner DK et al., Quality Control in Human In Vitro Fertilization. Seminars in Reproductive Medicine Vol.23, No. 4, pp.319-324, 2005
3. Sunde A et al., Time to take human embryo culture seriously. Human Reproduction, Vol.31,No.10, pp.2174-2182, 2016
4. Swain J. Is there an optimal ph for culture media used in clinical IVF?. Human Reproduction Update Vol. 18, No. 3, pp.333-339, 2012
5. Hentemann M et al., Differentail ph in embryo culture. Fertility and Sterility Vol. 95, No.4, pp.1291-1294, 2011
6. Swain J. Optimizing the culture environment in the IVF laboratory: ,impact of ph and buffer capacity in gamete and embryo quality. Reproductive Biomedicine Online Vol. 21, No. 1, pp.6- 16, 2010
7. Pool TB. An Update on embryo culture for human assisted reproductive technology: Media, performance and safety. Seminars in Reproductive Medicine Vol.23, no. 4, pp.309-318, 2005
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