Page 153 - PCC02

Practical Questions adressed to Commercial Medium Company-I
pH- and CO2 % related questions
• What is the pH of the media after equilibration in the incubator?
• How do you check pH?
• What is the CO2% of the incubator?
• How do you check CO2%?
• How often do you calibrate your incubators?
• How often do you calibrate your pH-meters?
• How do you calibrate pH meters/incubators?
• How often is the device used for measuring CO2% calibrated?
• What types of pH buffers are used? How often do you change pH buffers?
• Questions asked by customers
• What is most important (safest) to measure, pH in
the media or CO2 inside the incubator?
• Can I use the media after the expiry date is passed?
• Can I aliquot media into smaller volumes?
• How long before use should we prepare dishes?
• What is the optimal medium volume for droplets under oil in relation to number of embryos?
• The embryos cleave very slowly, what could have happened?
• Questions the company normally ask to customers that turn to us for trouble-shooting
• Products used and handling of products (media, dishes etc.)
• • What type of oil is used?
• • Do you aliquot media?
• • What type of plastic ware is used in the laboratory?
• • Do you rinse plastic ware and catheters before use?
• • For how long do you keep media bottles after they were originally opened?
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General questions not related with specific product composition
• Gas/pH; how and when to test? How is stage-specific pH achieved in culture media
I only have 1 incubator and would like to know the pH range I should run the incubator at if I have Fert, Cleav and Blast move through the same incubator. What would you recommend for a situation like this?
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How is target pH set in the first place for various types of media? (i.e. fert –cleav and blast) How is the target pH calculated?
When should I test pH?
Culture density individually or in groups?
How much medium should I use for culture and transfer?
What is best single or group culture?
Oil overlay
Why is an oil overlay needed? When should I use oil overlay? How much oil should I use? How should I store the oil?
Temperature
How long time does it take to temperature equlibrate media?
Why is temperature stability so important?
How to control the temperature when working outside the incubator?
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PRECONGRESS COURSE 02 I BARCELONA, SPAIN – 1 JULY 2018 153