Page 137 - PCC02

Kirkeegard F&S 2013
Ammonium
• AA are abundant in the fluids of the female reproductive tract (Harris 2005) ad hence are key components of mammalian embryo culture (Gardner 2008)
• AA fill key physiological niches as development and differentiation proceed ,including;
Acting as osmolytes (Baltz 2001)
Buffer of internal pH (Edwards 1998)
Regulation of carbohydrate metabolism (Lane &Gardner 2005)
• Thus inclusion of AA in modern culture media contributed to the increase of success for human IVF over the past two decades(Devreker 2001, Gardner 2008)
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