Page 54 - PRECONGRESS COURSE 03

Gene Ontology of deregulated genes in Fmn 2 -/- oocytes Under‐expressed in Fmn2 ‐/‐
01234
Chromosome segregation Cell cycle Protein Ubiquitination
Targeting to organels Organel organization Gene regulation
Gene transcription RNA biosynthesis
Metabolism Biosynthetic processes
Over‐expressed in Fmn2 ‐/‐
Translation Peptid biosynthesis
Biosynthetic processes Metabolism
01234
Fold enrichment
Functions associated: - meiotic divisions
– early development
down in
Fmn2 -/-
up in
Fmn2 -/-
RT-qPCR
8 6 4 2 0
Fmn2 +/- * Fmn2 -/-
Fmn2 Acp1Tcstv1 Tsc1H2eb1Cdc5L
Validation of the transcriptomic data by RT-qPCR
Transcriptomic analysis
Gene
Function
Ratio Fmn2-/- / Fmn2+/-
padj
Fmn2
Actin nucleator
0,043
4,03E- 171
Acp1
Tyrosine phosphatase
0,41
2,06E-14
Tcstv1
ZGA
0,11
1,46E-28
Tsc1
Wnt/beta-catenin pathway, cell proliferation
0,46
9,89E-10
H2eb1
MHC component
5,4
7,51E-16
Cdc5L
Splicing factor
9,3
3,17E-46
**
** * ** *
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Relative mRNA quantity