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When to perform ICSI
• TESE
• Severe male factor (?)
• Immunological male infertility (?)
• No fertilization with regular IVF
Cut‐off threshold for nl fertilization: >= 20 spermatozoa bound to ZP (Franken et al., F&S, 1993)
Number of sperm inseminated
Average number of sperm bound to HZ
500
12
1000
30
2000
31
5000
46
10000
77
20000
102
Boshoff & Huyser, Pretoria, 2015
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 57
