Page 143 - PRECONGRESS COURSE 01

Dept. of Experimental and Clinical Biomedical Science “Mario Serio”
Other collaborators
Baldi Group (Sperm Biology): Monica Muratori
Sara Marchiani
Lara Tamburrino
Marta Cambi
Semen analysis:
Erminio Filimberti Selene Degl’Innocenti Maria Grazia Fino
Clinical Studies:
Francesco Lotti Mario Maggi
Tecnobios Riproduzione ‐ Bologna
Andrea Borini Nicoletta Tarozzi Marco Nadalini
Department of Pediatrics,
Anna Meyer Children's University Hospital University of Florence
Chiara Azzari
References (alphabetical order)
Agarwal A, Majzoub A, Esteves SC, Ko E, Ramasamy R, Zini A. Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Transl Androl Urol. 2016 Dec;5(6):935‐950. doi:10.21037/tau.2016.10.03. Review.
Aitken RJ, Finnie JM, Muscio L, Whiting S, Connaughton HS, Kuczera L, Rothkirch TB, De Iuliis GN. Potential importance of transition metals in the induction of DNA damage by sperm preparation media. Hum Reprod. 2014 Oct10;29(10):2136‐47. doi: 10.1093/humrep/deu204.
Cissen M, Wely MV, Scholten I, Mansell S, Bruin JP, Mol BW, Braat D, Repping S, Hamer G. Measuring Sperm DNA Fragmentation and Clinical Outcomes of Medically Assisted Reproduction: A Systematic Review and Meta‐Analysis. PLoS One. 2016 Nov 10;11(11):e0165125. doi: 10.1371/journal.pone.0165125.
Esteves SC, Sánchez‐Martín F, Sánchez‐Martín P, Schneider DT, Gosálvez J. Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm. Fertil Steril. 2015 Dec;104(6):1398‐405. doi: 10.1016/j.fertnstert.2015.08.028.
Ghaleno LR, Valojerdi MR, Janzamin E, Chehrazi M, Sharbatoghli M, Yazdi RS. Evaluation of conventional semen parameters, intracellular reactive oxygen species, DNA fragmentation and dysfunction of mitochondrial membrane potential after semen preparation techniques: a flow cytometric study. Arch Gynecol Obstet. 2014 Jan;289(1):173‐80. doi: 10.1007/s00404‐013‐2946‐1. Kopeika J, Thornhill A, Khalaf Y. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Hum Reprod Update. 2015 Mar‐Apr;21(2):209‐27. doi: 10.1093/humupd/dmu063. Review.
Lotti F, Tamburrino L, Marchiani S, Muratori M, Corona G, Fino MG, Degl'Innocenti S, Forti G, Maggi M, Baldi E. Semen apoptotic M540 body levels correlate with testis abnormalities: a study in a cohort of infertile subjects. Hum Reprod. 2012 Dec;27(12):3393‐402. doi: 10.1093/humrep/des348.
Marchiani S, Tamburrino L, Maoggi A, Vannelli GB, Forti G, Baldi E, Muratori M. Characterization of M540 bodies in human semen: evidence that they are apoptotic bodies. Mol Hum Reprod. 2007 Sep;13(9):621‐31.
Marchiani S, Tamburrino L, Olivito B, Betti L, Azzari C, Forti G, Baldi E, Muratori M. Characterization and sorting of flow cytometric populations in human semen. Andrology. 2014 May;2(3):394‐ 401. doi: 10.1111/j.2047‐2927.2014.00208.x.
Meamar M, Zribi N, Cambi M, Tamburrino L, Marchiani S, Filimberti E, Fino MG, Biggeri A, Menezo Y, Forti G, Baldi E, Muratori M. Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus‐indica. Fertil Steril. 2012 Aug;98(2):326‐33. doi: 10.1016/j.fertnstert.2012.05.001.
Muratori M, Maggi M, Spinelli S, Filimberti E, Forti G, Baldi E. Spontaneous DNA fragmentation in swim‐up selected human spermatozoa during long term incubation. J Androl. 2003 Mar‐ Apr;24(2):253‐62.
Muratori M, Marchiani S, Tamburrino L, Cambi M, Lotti F, Natali I, Filimberti E, Noci I, Forti G, Maggi M, Baldi E. DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters. Fertil Steril. 2015 Sep;104(3):582‐90.e4. doi: 10.1016/j.fertnstert.2015.06.005.
Muratori M, Marchiani S, Tamburrino L, Tocci V, Failli P, Forti G, Baldi E. Nuclear staining identifies two populations of human sperm with different DNA fragmentation extent and relationship with semen parameters. Hum Reprod. 2008May;23(5):1035‐43. doi: 10.1093/humrep/den058.
Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E. Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress. Mol Med. 2015 Jan 30;21:109‐22. doi: 10.2119/molmed.2014.00158.
Muratori M, Tarozzi N, Cambi M, Boni L, Iorio AL, Passaro C, Luppino B, Nadalini M, Marchiani S, Tamburrino L, Forti G, Maggi M, Baldi E, Borini A. Variation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques: A Possible New Male Predictive Parameter of Pregnancy? Medicine (Baltimore). 2016 May;95(20):e3624. doi: 10.1097/MD.0000000000003624.
Osman A, Alsomait H, Seshadri S, El‐Toukhy T, Khalaf Y. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta‐analysis. Reprod Biomed Online. 2015 Feb;30(2):120‐7. doi:10.1016/j.rbmo.2014.10.018. Review.
Ribas‐Maynou J, García‐Peiró A, Fernández‐Encinas A, Abad C, Amengual MJ, Prada E, Navarro J, Benet J. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology. 2013 Sep;1(5):715‐22. doi: 10.1111/j.2047‐2927.2013.00111.x.
Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, Kirkman‐Brown J, Coomarasamy A. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta‐ analysis. Hum Reprod. 2012 Oct;27(10):2908‐17. doi: 10.1093/humrep/des261. Review.
Simon L, Zini A, Dyachenko A, Ciampi A, Carrell DT. A systematic review and meta‐analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl. 2017 Jan‐Feb;19(1):80‐90. doi: 10.4103/1008‐682X.182822. Review.
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