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KEY POINTS FOR HAVING FEMALE GAMETES FROM PLURIPOTENT STEM CELLS Peg3 gene
0% methylated
75% methylated
100% methylated
•mESC transfected with gcOCT4-GFP.
•Observation of different stages of GC development characterized by VASA, DMC1 and SCP3 expression
•By Day 12 of culture aggregates formed well organized structures similar to ovarian follicles. Most of them degenerate but around 20% give rise to “oocytes”> 40 μm.
• The functional activity of the follicles is evidenced by estradiol production
•All ZP markers are + except ZP1 (accounting for fragile ZP structure)
•At about day 43, structures similar (morphology and markers) to preimplantation embryos are found.
•These blastocyst-like structures represent parthenotes (probably) induced by culture conditions
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