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 Contd
Conclusions
• Semenwashingappearstosignificantlyreduceriskof transmission in HIV discordant couples desiring children, regardless of viral suppression in male partner.
• There are no studies to assist fertility with ART from low- income countries, esp. those with a large burden of HIV.
• Continueddevelopmentoflow-costsemenwashing and assisted reproduction technologies will be very helpful low resource countries.
Contd
Duru Shah
 Contd
Conclusions
• Spermwashingbasedontriplegradientspermselection coupled with extensive centrifugations is a highly reliable technique for HIV removal.
• UseofwashedspermsinbothIUI&IVFcycles maintains high pregnancy rates in assisted reproduction cycles.
• Despite extensive personnel training and effectiveness of the washing protocol, post-wash HIV test on semen is recommended to identify residual positive samples.
Duru Shah
Contd
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