Page 181 - ESHRE2019

The Rabbit Model: Results
•
•
Rates on coatings were not impaired:
• Mean value 89.4±3.1%,
comparable to
control conditions.
Severely impaired on all hydrogels!
• averaging around 27.6±26.4%.
Normal embryo development on synchronous coating
In vitro development on coatings and hydrogels Hatching/Hatched rates
Hydrogel conditions excluded in further analysis.
Impaired growth and development on Matrigel hydrogel
0h 24h 48h
Innovation
(Campo et al., 2019)
Foundation
35
The Rabbit Model: Results
Morphokinetic analysis and mRNA expression Embryo diameter
mRNA expression of three core pluripotency factors
• NANOG mRNA expression unaffected.
• Similar trend to morphokinetic analysis:
• SOX2 transcript abundance higher in NSC, SC and C+FBS.
• Similar OCT4 abundance in MC, SC and C+FBS Foundation
Innovation
• Comparable between SC and C+FBS.
• Significantly different to NSC and C‐FBS.
• Smallest average diameter in MC.
conditions.
(Campo et al., 2019)
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PRECONGRESS COURSE 11 I VIENNA, AUSTRIA – 23 JUNE 2019 179