Page 169 - ESHRE2019

Uterine bioengineering: emulating native tissues
Decellularization of tissues and organs •Decellularization (DC): removal of cells.
• Removal of epitopes responsible for chronic rejection (foreign cells/DNA).
No rejection of scaffold, all allogeneic donors can be used.
• While conserving ECM with native growth factors.  ECM is generally conserved between
species: xenogeneic donors.
• Easy storage of acellular scaffold (creation of
biobanks).
• Recellularization natural scaffolds with
Methods of decellularization
(Badylak et al., 2011)
Innovation
(autologous) stem cells:
• Three‐dimensional ultrastructure, surface topology
and composition provide “zip codes” for cells.
• Tissue sections (limited in size).
• Whole organs.
Foundation
11
Uterine bioengineering: emulating native tissues
Decellularization of tissues and organs
Whole organ decellularization Added bonus: vascularized scaffold.
Vasculature used for de- and recellularization. Delivery of DC agents, cells and
nutrients/oxygen.
Theoretically alleviating all transplantation-
related issues.
Innovation
(Ott et al., 2014)
Foundation
12
(Tapias and Ott, 2014)
New field of interest in reproductive medicine.
Badylak et al,, 2012
164
PRECONGRESS COURSE 11 I VIENNA, AUSTRIA – 23 JUNE 2019 167