Page 150 - ESHRE2019

Plasma metabolomics in search for biomarkers of endometriosis
Study
Method
Group
Findings
Diagnostic accuracy
Vouk et al. 2012
ESI‐MS/MS AbsoluteIDQTM p150 kit
40 OE patients, 52 healthy women
8 metabolites with significant changes:
sphingomyelins: SMOH C16:1, SM C16:1, SMOH C22:2  phosphatidylcholines:
PCae C34:0, PCae C32:2, PCae C30:0, PCae C34:2, PCae C36:1
Model (SMOH C16:1, PCaa C36:2/PCae C34:2, age, BMI) SEN: 90.0%
SP: 84.3% AUC: 0.94
Letsiou et al. 2016
UPLC‐MS/MS (SteroIDQ kit), UPLC‐ESI‐Q‐ TOF
Patients with pelvic pain or infertility 29 E (I‐ IV), 19 controls, luteal and folicular phase
 lauroylcarnitine, oleylcarnitine, myristoylcarnitine, tetradecenoylcarnitine, hexadecenoylcarnitine
 trimethylamine‐N‐oxide
PLS‐DA model
SEN: 81.8% SP: 88.9% PPV: 75%
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Metabolomics of peritoneal fluid and endometrium
Study
Sample
Method
Group
Findings
Diagnostic accuracy
Vouk et al. 2016
PF
ESI‐
MS/MS
Absolute IDQTM
p150 kit
29 OE patients, 36 healthy women
10 metabolites with significant changes:
carnitine and acylcarnitines, sphingomyelins, phosphatidylcholines.
Model (C0/PCae C36:0, PCaaC30:0/ PCaeC32:2, age) SEN: 82.8%
SP: 94.4% AUC: 0.94
Li et al. 2018
endome ‐trium
UHPLC‐ ESI‐ HRMS
29 patients (I, II), 37 infertile controls, folicular phase
hypoxantine, Arg, Tyr, Leu, Lys, inosine, arachidonic acid, guanosine, xanthosine, lysophoshatidylethanolamine, Asp
uric acid
Model (uric acid, hypoxantine, lysophospatidyl‐ ethanolamine) SEN: 66.7%
SP: 90.0% AUC: 0.87
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