Page 128 - ESHRE2019
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Experimental evidence
of the role of filopodia in compaction
• Microsurgical laser ablation of filopodia causes loss of elongated cell shape
• Molecular disruption of the filopodia components • E‐cadherin, α‐ and β‐catenin
• F‐actin
• myosin‐X
prevents cells from elongating and embryo from compacting
Filopodia formation is highly regulated
Filopodia are retracted prior to cell division
• Blastomeres never extend reciprocal filopodia on top of each other
• Blastomeres receiving filopodia never divide when filopodia are extended over their membranes
• 70% of blastomeres with filopodia undergo symmetric divisions (implications for the balance between ICM and trophectoderm populations)
Fierro‐Gonzales et al., 2018
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