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In clinical practice
• Detecting risk factors for increased sperm DNA fragmentation
• Measuring sperm DNA fragmentation index
• Eliminating risk factors
• Antioxidant treatment
• Checking DNA fragmentation index before ART
• Selecting sperms with lowest DFI for ART.
Selecting sperms with lowest DFI forART.
• In case of high and variable DNA fragmentation: Semen collection soon after last ejaculation
• Gradient density selection
• 1 aliquot: Sperm DNA fragmentation measurement
• Cryopreservation of remaining spermatozoa
• Repeat procedure at least 3 times weekly
• ICSI with cryopreserved spermatozoa with lowest sperm DNA fragmentation.
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