Page 115 - Eshre 2018

FOLLICULAR VIABILITY AND DENSITY ASSESSMENT USING NEUTRAL RED STAINING
 Neutral Red is a weak cation that passes through the intact plasma membrane and becomes concentrated in lysosomes of viable cells (Triglia et al., 1991).
(Chambers et al., 2010, Hum Reprod)
Human ovarian cortex stained with Neutral Red
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Frozen/thawed ovine cortical tissue stained with Neutral Red for 4 hours
SIMPLE PROTOCOL FOR NEUTRAL RED STAINING IN SITU
1-2 pieces of thawed ovarian cortex
Ovarian cortex chopped into smal pieces (<100μm) with the McIIwain Tissue Chopper
Incubated for 3-4 hours in Neutral Red (15μl NR per 1 ml of prewarmed and CO2 equillibrated culture media)
(Kristensen SG et al., Hum Reprod, 2011)
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PRECONGRESS COURSE 16 I BARCELONA, SPAIN – 1 JULY 2018 115