Page 105 - Eshre 2018

TRANSPORTATION OF OVARIAN TISSUE WITHIN FERTIPROTEKT
 The ovarian tissue is transported in a tube filled with an organ perfusion solution (Custodiol) or a phosphate-buffered saline (PBS)solution.
 Overnight transportation are carried out in special isolated transportation containers with precise temperature documentation.
 The mean period between harvesting and final cryopreservation is 18 hours to a max. of 24 hours (Van der Ven H. et al., Human Reproduction, 2016).
 FertiPROTEKT has so far published 17 live births.
FOLLICLE VIABILITY FOLLOWING OVERNIGHT/PROLONGED TRANSPORT OF OVARIAN TISSUE
 First case of prolonged transport was reported by Rosendahl M. et al. 2011. Viable follicles were present after 20 hours of transport on ice.
 Isachenko et al., showed that exposing human cortical tissue for suprazero temperatures for 0–26 hours did not inhibit the development of follicles during subsequent in vitro culture (Isachenko E. et al., Fertil Steril 2009).
 Effect of cooling on bovine ovarian tissue (Lucci CM et al., Theriogenology, 2004).
 Storage of ovarian tissue at 4 °C for up to 18 hours kept the percentage of normal follicles similar to controls.
 Storage of ovarian tissue at 20 °C for 18 hours significantly reduced the percentage of morphologically normal follicles compared to controls.
 The type of solution (saline or coconut water) that the ovaries were immersed in had little effect on the results.
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PRECONGRESS COURSE 16 I BARCELONA, SPAIN – 1 JULY 2018 105