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Genome editing mechanisms
Creation of a Double Strand Break (DSB)
• Meganucleases
• Engineered Zinc-finger nucleases (ZFNs)
• Transcription activator-like effector
nucleases (TALENs)
• CRISPR/Cas9 (Clustered Regularly
Interspaced Short Palindromic Repeats) Fixation by cell repair mechanisms
• •
NHEJ: Non-homologous end-joining
HDR: Homology- directed repair
PCC Genetics - ESHRE BCN 2018
Genome editing applications
A.Target gene mutation
B. Model disease mutations (Generation of knockin/knockout animal models)
C.Modify gene expression in cell lines (activation or silencing)
D.Study gene function (gene labelling for cell lineage tracking, ...)
E. Create transgenic organisms F. Synthetic biology
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