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Proposed sequence of selfish events in the human testis
Testicular tumour (SpT)
mutant sperm
Are these MAGE-A4 positive tubules carrying selfish mutations?
Maher et al, Andrology 2013
Can we find selfish mutations in MAGE-A4 positive tubules?
Section 02_MAGEA4 Section 04LCM_Methyl green Section 06_MAGEA4
Laser Capture Microdissection (LCM)
Targeted re-sequencing of >100 candidate genes HaloPlexTM and Illumina Sequencing
FGFR1, FGFR2, FGFR3, RET, HRAS, KRAS, NRAS, BRAF, MAP2K1, MAP2K2, SHOC2, CBL, PTPN11 ............
Page 33 of 102
Maher et al, PNAS 2016
ProtK/DNA extraction Whole Genome Amplification
PRECONGRESS COURSE 08 I BARCELONA, SPAIN – 1 JULY 2018 39
