Page 86 - Eshre 2018
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Learning objectives
What is genome editing and what it does? Germline genome editing
Human embryo genome editing Indications for genome editing in human reproduction
CRISPR/Cas9
2005 ‐ 2009 (Mojica, 2005) bacteria have a mechanism that directs nucleases, such as Cas9, to bind and cut invading DNA that matches “guide RNAs” encoded in bacterial genome regions containing short repeats (CRISPR).
2010 and 2011: definition of the critical components of the CRISPR‐Cas9 system 2012: same results confirmed in vitro
2013: modifications of the CRISPR‐Cas9 system to work in mammalian cells.
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