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CONCLUSIONS
• The very recent finding that SSCs isolated from adult human testis and in vitro co-cultured with somatic testicular cells differentiate to spermatids opens new possibilities in the field of reproductive and regenerative medicine
• SSC isolation, propagation, preservation and autotransplantation is a potential strategy to restore fertility in prepubertal patients undergoing chemiotherapy
• The derivation of functional sperm from patient- specific iPSCs is a challenge for the future
Bibliography
Histological and histopathological evaluation of the testis Eds: L. Russell, R.A. Ettlin, A.P. Shina Hikim, E.D. Clegg; 1990, Cache River Press
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. Sci Rep. 2017 Nov 13;7(1):15459. doi: 10.1038/s41598-017-15799-3
In Vitro Spermatogenesis in Explanted Adult Mouse Testis Tissues.
Sato T, Katagiri K, Kojima K, Komeya M, Yao M, Ogawa T. PLoS One. 2015 Jun 12;10(6):e0130171. doi: 10.1371/journal.pone.0130171.
In vitro production of functional sperm in cultured neonatal mouse testes.
Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T. Nature. 2011 Mar 24;471(7339):504-7. doi: 10.1038/nature09850
Offspring production with sperm grown in vitro from cryopreserved testis tissues.
Yokonishi T, Sato T, Komeya M, Katagiri K, Kubota Y, Nakabayashi K, Hata K, Inoue K, Ogonuki N, Ogura A, Ogawa T. Nat Commun. 2014 Jul 1;5:4320. doi: 10.1038/ncomms5320.
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