Page 69 - Eshre 2018
P. 69
Nature, 2011
• 2.5 dpp mouse pups
• alfaMEM + 10% KSR (invitrogen) or 40 mg/ml AlbuMAX
• meiosis and haploid cell GFP-markers for monitoring the progress of spermatogenesis in vitro
• sperm formation after 42-45 days of culture • ROSI or ICSI
• normal fertile offspring
In vitro spermatogenesis in the mouse ( Boitani et al., unpub.)
T0
Sertoli Cells Leydig Cell
Gonocytes
First week
Sertoli cells Third week
Zygotene Spermatocytes
Leydig Cell
Pachytene spermatocytes
Spermatogonia
10μm ___
10μm ___
Zygotene ___ 10μm
Second weekZygotene Spermatocytes
Mid-pachytene spermatocytes
spermatocytes
Early pachytene spermatocytes
Spermatogonia
10μm ___
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