Page 125 - Eshre 2018

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miRNA as a diagnostic tool infertility
Spermatozoa
Testicular tissues
Embryonic culture media
Normozoospermia
Mixed Atrophy
Oligoasthenozoospermia
Normozoospermia
Negative Pregnancy
Positive Pregnancy
S
e
G
lA
G
e
r
e
e
r
Asthenozoospermia
Oligoasthenozoospermia
S
r
t
r
m
to
o
l
l
i
mC
i
-
-
C
C
C
e
el
e
e
l
l
l
A
r
l
l
l
-
-
O
O
n
r
r
r
e
n
l
es
l
y
y
st
t
N
N
o
o
r
r
m
ma
al
lt
ti
i
s
s
s
su
u
e
e
Seminal Plasma-derived extracellular microvesicles
25.05.2018
Prof. Dr. Eckart Meese
Seite
9
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Part I: Materials and Methods
Overview of the design strategy
Spermatozoa
A
A
s
s
t
t
h
he
e
n
no
o
z
z
o
o
o
o
s
sp
p
e
er
r
m
m
i
i
a
a
O
O
l
li
ig
g
o
oa
a
s
s
t
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h
h
e
en
no
oz
z
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os
s
p
pe
er
rm
mi
i
a
a
Total RNA isolation
RNA Assessment
miRNA microarray
Real Time PCR
Data analysis
Normozoospermia
Objective: To determine whether microRNAs are differentially expressed in men with normal versus impaired spermatogenesis. and to find a biomarker for accurate diagnosis of male infertility.
01.07.2018
Prof. Dr. Eckart Meese Seite
10
Page 119 of 130
PRECONGRESS COURSE 01 I BARCELONA, SPAIN – 1 JULY 2018 125