Page 28 - PRECONGRESS COURSE 01

Analytical aspects
Source of error Remedy
– Low aliquot volume (<50 μL)
– Risk for poor representativity
– Inexact volume due to viscosity
– Influence of random error
– Inexact volume due to preparation depth
– Replicate assessment with comparison
– Positive displacement pipetter
– 400 observations = ±10% error
– 100 μm haemocytometer
ESHRE-SIGA PreCongress Course 9 July 2, 2017
Low volume – less representative
– The ejaculate is not homogenous
– Sequential emptying of glands
– Different viscosities
– Macroscopical mixing doesn’t mean microscopical mixing
– Low volume aliquots (3-5 μL) very seldom representative
– Sub compartments with more or less spermatozoa
– WHO recommendation is at least 50 μl for concentration assessment
– For motility (10 μL) replicate aliquots for assessment compulsory
ESHRE-SIGA PreCongress Course 10 July 2, 2017
28
Page 24 of 175