Page 131 - PRECONGRESS COURSE 01

Some considerations
• Different methods to evaluate sDF give variable and incomparable results (average sDF values in different studies vary extensively depending on the method of evaluation, other than patients’ selection, female factor)
• There is no consensus on which is the “gold standard” method
• Lack of standardization of these methods with the exception of
SCSA
• Several cutoffs (depending on the study, method, patients selection etc)
• Indirect methods (SCSA or SCD) evaluate chromatin susceptibility to damage
• Direct methods (Comet, TUNEL) evaluate the real damage (single and double DNA strand breaks)
• Which of these assays differentiate clinically important damage from unsignificant damage? Results of metanalyses where studies have been grouped according to the method
Miscarriage rates in natural and assisted reproduction
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PRECONGRESS COURSE 01 I GENEVA, SWITZERLAND – 2 JULY 2017 131